Colonnes SEC Analytiques, Préparatives
Unix™, Unix™-C, Zenix®/ Zenix®-C, SRT®/ SRT®-C, SRT®-10/ SRT®-10C, et Nanofilm® sont neuf types de colonnes de chromatographie SEC basées sur un revêtement de surface innovant et une technologie de particule uniforme, hydrophilique, et neutre le film d’une épaisseur du nanomètre est greffé chimiquement sur des particules de silice de haute pureté mécaniquement stabilisées de 1.8 µm, 3 µm, 5 µm, ou 10 µm.
Utilisant deux technologies uniques de revêtement de surface monocouche debout sur silice poreuse pour UNIX ™, Zenix ®, SRT ® et SRT ®-10; et monocouche couchée sur silice poreuse pour UNIX ™-C, Zenix ®-c, SRT ®-C, et SRT ®-10C, Sepax offre des choix de chimie de surface basés sur le type d'échantillon et l'hydrophobicité de l'échantillon afin d'obtenir une séparation performante et de haute résolution.
Nanofilm® est constitué d’une phase stationnaire spéciale - un revêtement polymère brossé.
Un tel revêtement polymère hydrophile neutre convient pour les protéines chargées positivement et les protéines hydrophobes, telles que les protéines membranaires.
- Haute efficacité et résolution
- Extrême stabilité en solution à forte concentration en sels
- Grande reproductibilité
- Séparation de protéines à faible concentration en sels
- Taux de récupération des protéines élevé et conservation de l'activité biologique
- large gamme de pH applicable 2 - 9
Droite de calibration des protéines avec phases Nanofilm SEC-150, Nanofilm SEC-250, et Nanofilm SEC-500.
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Applications
| (Nanofilm Packings | Limite d'exclusion Protéines MW |
|---|---|
| Nanofilm SEC-150 | 750,000 |
| Nanofilm SEC-250 | 1,250,000 |
| Nanofilm SEC-500 | 2,500,000 |
Antibody (MAb)
Analytical Characterization of NIST MAb by Sepax SEC, HIC, RP and CEX Analytical Characterization of NIST MAb by Size Exclusion Chromatography EM1006 - Bispecific mAb SEC analysis Sepax SEC Analytical Column Loading Capacity Reduced MAb Separation on Zenix SEC-300 2.1x300 with LC/MS Volatile Buffer IgE and IgG Separation on Zenix SEC-300 Fast MAb Analysis on Zenix SEC - Flow rate MAb Half Body TCEP Disulfide reduction High Loading of MAb Fragments on Zenix 300 Fresh MAb Analysis on Zenix 300 MAb Fragments Separation and Characterization by SEC and SEC/MS MAb Heavy chain and Light Chain Separation by Zenix SEC Fast Separation of MAb and its Aggregates in Less Than 3 Mins Reproducibility for MAb Analysis on Zenix SEC MAb High Resolution by Zenix SEC Fast MAb analysis by Zenix SEC BevacizuMAb and Erbitux Antibodies by Zenix SEC-300 MAb Fragments Analysis on Zenix SEC-150 IgG on Zenix SEC- Pore SelectionAntibody and Biosimilar
Cell Lysate
E. Coli Lysate separation on SEC, SAX and RP Separation of E. Coli Lysate on Various Zenix Pore SizesDNA/RNA
Fusion Protein
Glycoprotein
rhEPO (CHO cell) Separation on Zenix SEC-150 Intact Glycoprotein Isoforms Separation on Proteomix SAXHeparin
Membrane Protein
Others
PEG/PEGylated Protein
PEG/PEGylated Protein and Polymer
Peptide
Zenix and Zenix-C SEC-80 for Peptide Analysis Separation of 13.7 KD to 120 Da Peptide on Zenix-80 Four Peptide Mixture from 1kD to 6kD on Zenix SEC-80 Separation of Asparaginase on Zenix SEC-150 Competition Comparison for Peptide Separation on SEC Analysis of 5kD and 8kD Peptides by Zenix SEC-150 Analysis of Peptide Mixture on Zenix SEC -100 Separation of Peptides and Small Proteins using Zenix SEC-100Peptide and Cell Lysate
E.Coli Tryptic Digest on Zenix SEC-80 Peptide Separations Using Zenix SEC-80 Analysis of E.coli Tryptic Digest on Zenix SEC-80Polymer
Water Soluble Polymer Separation on SEC Columns Tremella Polysaccharide Analysis on Zenix SEC Polystyrene Sulfonate Polymer Separations on SEC PEG Separation on two Zenix Columns in TandemProtein
SEC-300 Overlay with Different Particle Sizes -QC standard overlays with 3, 5, 10 mm SEC-300, 7.8 x 300 mm Complex Protein Sample Mixtures Analysis on Sepax Columns Life Time Test of Zenix 300 with High Salt at pH 8.5 Fast Assay Application on Zenix-300 Protein Separation on Zenix SEC-300 with HPLC and FPLC system Lysozyme Elution Profiles on Zenix Light Scattering Detection of BSA on Zenix SEC-300 Fast Separation of Horse Serum by Zenix 3 & 5 µm SEC on Separation Efficiency Improved Resolution on 3 µm SEC MW Calibration Curve on Zenix Phases Separation of Bio-Rad Protein Mixture by Zenix Separation of Protein Mixture by Zenix Phases Separation of Proteins and Peptide on Zenix MixtureProtein and PEG/PEGylated Protein
Unix™, Unix™-C
Colonnes SEC UHPLC 1.8 µm
Zenix®/ Zenix®-C
Colonnes SEC HPLC haute résolution
SRT®/ SRT®-C
Colonnes SEC HPLC
SRT®-10/ SRT®-10C
Colonnes SEC Préparatives et Bulk
KITS de Screening SEC
| P/N | Dimension |
| SECKIT-7830 | 7.8×300mm |
| SECKIT-4630 | 4.6×300mm |
Le kit de sélection de phase SEC comprend nos deux colonnes SEC les plus vendues: une colonne Zenix ™ 300 Â et une colonne Zenix ™ -C 300 Å. Les colonnes Zenix et Zenix-C 300 Å 3 µm combinées offrent deux chimies de revêtement de surface uniques, idéales comme outil de criblage pour les chercheurs de MAb et de Protéines à la recherche de la chimie de revêtement optimisée et spécifique pour chaque échantillon. Le Kit comprend des données de profil de séparation sur divers types d’échantillons tels que MAb, PEG, standards BioRad, etc.
Applications
ADC
Antibody (MAb)
Fast Monoclonal Antibody Titer Determination Followed by SEC Aggregation Analysis Analytical Characterization of Monoclonal Antibody IgG2 MAb Separation on Unix-C SEC-300 UHPLC ColumnFusion Protein
Protein, Nucleic Acid and Peptide
SEC Separation of Protein Mixtures on SRT and TSKgel Analysis of Insulin on SRT SEC-150 EPO Analysis on SRT SEC-300 Separation of Thyroglobulin and Aggregates on SRT-1000 Separation of Human Recombinant Protein on SRT-1000 Protein Separation Profiles on SRT-10 Phase The Column Length Impact on Separation Efficiency From Analytical to Semi-Prep Separation on SRT-10 Protein Separation in Organic Buffer on SRT Pore Size Selection on Lysate Separation on SRTAntibody and Monoclonal Antibody
Sepax SEC Analytical Column Loading Capacity MAb on SRT SEC - LMW & HMW regions MAb & IgG on SRT SECNatural and Synthetic Polymer
Separation of Polysaccharides on SRT Low MW Polysaccharides on SRT SEC-100 and 150 PEG Analysis on SRT-150 and SRT-300 Separation of Dextran on SRT-300 Separation of Pullulan on SRT-300Bacteria and Virus